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New Multidrug Efflux Inhibitors for Gram-Negative Bacteria

active efflux of antibiotics to prevent the accumulation of toxic intracellular concentrations them to contribute to a clinically relevant multidrug resistance. Potentiates the inhibitory activity of active efflux of antibiotics, suggesting that the efflux inhibitor can be used in combination with antibiotics to reverse drug resistance.

Expression rama by Salmonella enterica serovar Typhimurium increased in response to inhibition of efflux, regardless of the mode of inhibition. We hypothesize that the measure Rama promoter activity can act as a reporter for the inhibition of efflux. A quick, inexpensive, and high-throughput screen of green fluorescent protein (GFP) to identify inhibitors of efflux developed, validated, and implemented. Two compounds screened chemical libraries for compounds that increase the production of GFP. Fifty of the compound in the 1,200-Prestwick compound chemical library identified as potential inhibitors of efflux, including inhibitors of efflux previously marked mefloquine and thioridazine.

There are 47 168 107 hits on the library of proprietary compounds from L. Hoffmann La Roche; 45 confirmed hits, and dose response is determined. Coloring depletion and accumulation tests showed that 40 Roche and three Prestwick chemical library compounds are inhibitors of efflux. Most compounds have a combination of antibiotic efflux-inhibitor-specific and / or species-specific synergies in antibiotic disc diffusion and checkerboard test performed with Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Salmonella Typhimurium.

These data indicate that both narrow spectrum and broad-spectrum combination of inhibitors of efflux with antibiotics can be found. Eleven novel inhibitor compound efflux potential antibiotic activity against at least one species of Gram-negative bacteria, and the data revealed E. coli mutants with loss of function AcrB suggest that this is a Gram-negative bacteria Multidrug-resistant AcrB inhibitors.IMPORTANCE pose a serious threat to human health and animal.

Molecules that inhibit the multidrug efflux offers an alternative approach to address the challenges caused by antibiotic resistance, by potentiating the activity of old, licensed, and new antibiotics. We have developed, validated, and apply high-throughput screens and used to identify inhibitors of efflux of two libraries compound selected for high chemical and pharmacological diversity. We found that the new high-throughput screen is a valuable tool to identify inhibitors of efflux, as evidenced by 43 new efflux inhibitor described in this study.

 New Multidrug Efflux Inhibitors for Gram-Negative Bacteria
New Multidrug Efflux Inhibitors for Gram-Negative Bacteria

 

bioinformatics analysis of high throughput data for validating potential biomarkers and novel small molecule drugs for glioblastoma multiforme

Objective: There are no recent improvement in the results of glioblastoma multiforme (GBM), with an average remaining life of 15 months. As a result, the need to identify new biomarkers for diagnosis and prognosis of GBM, and to develop targeted therapies is high.

This study aims to develop biomarkers for GBM pathogenesis and prognosis.
Methods: In total, 220 overlapping genes differentially expressed (degs) is obtained by integrating four microarray datasets from the Gene Expression Omnibus database (GSE4290, GSE12657, GSE15824, and GSE68848). Then the 140-node network of protein-protein interactions with the 343 interaction is built.

Cortisol Enzyme Immunoassay kit - 5 Strip plates

K003-H5 5x96 well plate
EUR 1055

Cortisol Enzyme Immunoassay kit - 5 Whole plates

K003-H5W 5x96 well plate
EUR 1055

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST 1 mL
EUR 1106.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

SARS-CoV-2 Antigen Rapid Test Kit (Colloidal gold Immunoassay)

CoV2Ag-25 25 Tests/Kit
EUR 35
Description: This SARS-CoV-2 Antigen Rapid Test Kit (Colloidal Gold) uses the sandwich immunocapture method and colloidal gold immunochromatography to qualitatively determine the presence of SARS-CoV-2 antigens in human oropharyngeal swabs, nasal swabs and nasopharyngeal swabs. It is helpful as an aid in the screening of early mild, asymptomatic, or acute patients for identification of SARS-CoV-2 infection.

Human Estrone-3-Glucuronide (E1G)Enzyme Immunoassay ELISA Kit

QY-E05423 96T
EUR 361

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Colloidal gold immunoassay)

CP04010 25T/kit Ask for price
Description: The Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Serum/Plasma/Whole blood) is a qualitative membrane-based immunoassay for the detection of SARS-CoV-2 neutralizing antibodies in serum, plasma and whole blood. The sample is dropped into the sample well, and chromatography is performed under the capillary effect. The SARS-CoV-2 neutralizing antibodies in the sample combined with the colloidal gold-labeled SARS-CoV-2 spike protein(SP), then spread to the test area. It is captured by coated SP subunit RBDNTD-CTD, to form a complex and gather in the test area (T line). The quality control area is coated with mouse anti-chicken IgY, and the colloidal goldlabeled chicken IgY is captureed to form a complex and aggregate in hte quality control area (C line). If the C line does not show color, it indicates that the result is invalid, and this sample need to be tested again.

Immunoassay Stabilizer

85R-103 1 liter
EUR 481
Description: Stabilizing buffer for use in immunoassays

Cystatin C Immunoassay Kit

K012-H1 1x96 well plate
EUR 405

11,12-DHET Immunoassay Kit

DH4 1 Kit
EUR 307

Porcine PRL(Prolactin) ELISA Kit

EP0139 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.094 ng/ml

Rat Prl(Prolactin) ELISA Kit

ER0076 96T
EUR 476.25
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.469 ng/ml

Mouse PRL(Prolactin) ELISA Kit

EM0158 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Bovine PRL(Prolactin) ELISA Kit

EB0013 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 0.469 ng/ml

Chicken PRL(Prolactin) ELISA Kit

ECH6395 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Chicken;Sensitivity: 0.469 ng/ml

Goat PRL(Prolactin) ELISA Kit

EG0008 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Goat;Sensitivity: 18.75IU/ml

Human PRL(Prolactin) ELISA Kit

EH0259 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

Rabbit PRL(Prolactin) ELISA Kit

ERB0102 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.188 ng/ml

Sheep PRL(prolactin) ELISA Kit

ESH0063 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 0.188 ng/ml

Recombinant RAT Prolactin

P1986 100ug Ask for price
Description: Recombinant protein for RAT Prolactin

CaspSELECT Caspase 3 Immunoassay Kit

55R-1338 100 assays
EUR 1022
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

cAMP Direct Immunoassay Kit (Colorimetric)

K371-100
EUR 512

cGMP Direct Immunoassay Kit (Colorimetric)

K372-100
EUR 512

QuickTiter Adenovirus Titer Immunoassay Kit

VPK-109 100 assays
EUR 705
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells.

cAMP Direct Immunoassay Kit (Colorimetric)

K2044-100 100 assays
EUR 529

cGMP Direct Immunoassay Kit (Colorimetric)

K2066-100 100 assays
EUR 529

Mouse Prl3b1(Prolactin 3B1) ELISA Kit

EM1597 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Mouse Prl3d1(Prolactin 3D1) ELISA Kit

EM1598 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Mouse Prl8a8( Prolactin-8A8) ELISA Kit

EM0798 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 46.9pg/ml

Human PRLR(Prolactin receptor) ELISA Kit

EH15147 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Recombinant mouse Prolactin-3D1

P2793 100ug Ask for price
Description: Recombinant protein for mouse Prolactin-3D1

Immunoassay Stabilizer (BSA free)

85R-103BF 1 liter
EUR 481
Description: BSA Free Stabilizing buffer for use in immunoassays

Human PIP(Prolactin-inducible protein) ELISA Kit

EH2124 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

CaspSELECT? Caspase-3 Immunoassay Kit (Fluorometric)

K163-100
EUR 751

Human Albumin AssayLite Fluorescent Immunoassay Kit

FEA2201 96 Well Plate
EUR 396

Human Ceruloplasmin AssayLite Fluorescent Immunoassay Kit

FEC5201 96 Well Plate
EUR 396

Human Haptoglobin AssayLite Fluorescent Immunoassay Kit

FEH1003 96 Well Plate
EUR 396

Human IgA AssayLite Fluorescent Immunoassay Kit

FEI7001 96 Well Plate
EUR 396

Human IgG AssayLite Fluorescent Immunoassay Kit

FEI7200 96 Well Plate
EUR 396

Human IgM AssayLite Fluorescent Immunoassay Kit

FEI7301 96 Well Plate
EUR 396

Mouse Albumin AssayLite Fluorescent Immunoassay Kit

FEMA2201 96 Well Plate
EUR 396

Human Prealbumin AssayLite Fluorescent Immunoassay Kit

FEP2010 96 Well Plate
EUR 396

Rat Albumin AssayLite Fluorescent Immunoassay Kit

FERA2201 96 Well Plate
EUR 396

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Enzyme-linked immunosorbent assay)

CP04012 96T/5*96T Ask for price
Description: It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

(1-Kit )11,12-EET/DHET Immunoassay Kit

DH5 1 Kit
EUR 307

Mouse Pip( Prolactin-inducible protein homolog) ELISA Kit

EM0733 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Human PRLHR(Prolactin-releasing peptide receptor) ELISA Kit

EH15148 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Melamine Rapid Test Kit

abx092011-50tests 50 tests
EUR 370

NDV rapid test kit

RG15-03 1 box
EUR 139.05
Description: Please check the datasheet of NDV rapid test kit before using the test.

Nitric Oxide Synthase (NOS) enzyme assay, colorimetric micro assay kit, 96 test, Quantitative

1290-NOS-1 1 kit
EUR 651

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Human Complement C3 AssayLite Fluorescent Immunoassay Kit

FEC2101 96 Well Plate
EUR 396

Human Complement C4 AssayLite Fluorescent Immunoassay Kit

FEC2102 96 Well Plate
EUR 396

Human Fibrinogen (FBG) AssayLite Fluorescent Immunoassay Kit

FEF1040 96 Well Plate
EUR 396

QuickTiter Adenovirus Titer Immunoassay Kit, Trial Size

VPK-109-T 20 assays
EUR 374
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells.

Up-converting Phosphor immunoassay analyzer

abx095263-1Machine 1 Machine
EUR 6105

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Mouse Ide(Insulin-degrading enzyme) ELISA Kit

EM0694 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Human IDE(Insulin-degrading enzyme) ELISA Kit

EH1988 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml

Rat Ace(Angiotensin-converting enzyme) ELISA Kit

ER0094 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Mouse ECE1(Endothelin Converting Enzyme 1) ELISA Kit

EM0995 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Monkey ACE(Angiotensin I Converting Enzyme) ELISA Kit

EMK0001 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml

Human UBE2C(Ubiquitin Conjugating Enzyme E2C) ELISA Kit

EH3939 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Mouse ACE(Angiotensin I Converting Enzyme) ELISA Kit

EM0011 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

EH0026 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml

Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit

EH0027 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.234 ng/ml

Human UBE2I(SUMO-conjugating enzyme UBC9) ELISA Kit

EH1264 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human APEH(Acylamino-acid-releasing enzyme) ELISA Kit

EH1573 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human ECE1(Endothelin-converting enzyme 1) ELISA Kit

EH2454 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Rat Ace2(Angiotensin-converting enzyme 2) ELISA Kit

ER0609 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Rat IRE1(Inositol requiring enzyme 1) ELISA Kit

ER1646 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus ;Sensitivity: 28.1pg/ml

Rabbit ACE(Angiotensin I Converting Enzyme) ELISA Kit

ERB0001 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.188 ng/ml

Sheep ACE(Angiotensin I Converting Enzyme) ELISA Kit

ESH0001 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 0.469 ng/ml

Melamine (MEL) Rapid Test Kit

abx092057-50tests 50 tests
EUR 370

Clenbuterol (CLE) Rapid Test Kit

abx092058-50tests 50 tests
EUR 244

Ractopamine (RAC) Rapid Test Kit

abx092059-50tests 50 tests
EUR 244

Salbutamol (SAL) Rapid Test Kit

abx092060-50tests 50 tests
EUR 244

Tetracycline (TCs) Rapid Test Kit

abx092063-50tests 50 tests
EUR 398

Sulfonamides (Sas) Rapid Test Kit

abx092064-40tests 40 tests
EUR 398

Quinolones (QNs) Rapid Test Kit

abx092065-40tests 40 tests
EUR 398

Ciprofloxacin (CPFX) Rapid Test Kit

abx092066-50tests 50 tests
EUR 398

Quinolones (QNs) Rapid Test Kit

abx092067-40tests 40 tests
EUR 398

Brucella Antibody Rapid Test Kit

abx092069-40tests 40 tests
EUR 356

PCRAgH5 AIV Detection Test Kit

PD55-02 1 kit
EUR 902.47
Description: Please check the datasheet of PCRAgH5 AIV Detection Test Kit before using the test.

Rapid Leishmania Ab Test Kit

RB2104 1 box
EUR 127
Description: Please check the datasheet of Rapid Leishmania Ab Test Kit before using the test.


Results: The immune response and cell adhesion molecule that functions and pathways most significantly enriched, respectively, between degs. ITGB5 gene designated hub and RGS4, which has a high level of connectivity, is closely correlated with patient prognosis, and mining data bases GEPIA further confirmed differential expression in GBM compared to normal tissue. We also determined the 20 most appropriate small molecules that could potentially reverse GBM gene expression, Prestwick-1080 is the most promising and has the highest negative value.